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File:TMV.jpg

Summary

TMV Virus
Author: T. Moravec
Source: en:user:Xmort
Virus particles were purified by PEG/NaCl precipitation and sucrose cushion ultracentrifugation. Virus was then resuspended in 20 mM PO4 buffer, pH=7.2 to final concentration 10mg/ml. Formvar coated grids were floated on drops containing virus for 30 minutes, 3 times washed in deionised water and then stained with 2% PTA for 5 minutes.

ru: Вирус табачной мозаики ВТМ

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